Fig. 6
From: Stress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny
CS effects on zebrafish males in terms of sperm quality after approximately three rounds of spermatogenesis exposure. A Trial 4 design summary. Males were individually tagged 15 days before the beginning of the trial using visible implant elastomers (VIE). One day before the beginning of the CS protocol, males were divided into in homogeneous groups in terms of sperm quality parameters. After the 21-day CS protocol, on day 22, sperm samples were processed to evaluate the effect of chronic stress in the S+ group. B Sperm concentration, total motility, progressive motility, and fast cells fraction of the groups at day − 1. Mean values of C cell concentration, D total motility (%), and E progressive motility (%) after the trial 4 conclusion. F Before–after graphs for the experimental groups S− and S+ for the endpoints concentration, total motility, progressive motility, and fast cell subpopulation. S−: control males. S+: males exposed to the CS protocol. Data are presented as mean ± SEM (n = 9; fish batch 1). *p < 0.0500, **p < 0.0100, ***p < 0.0010, ns, not significant (p > 0.0500)