Fig. 7

AS1 is not inherently attractive. A A chemical attraction/aversion assay in which either vehicle (2% DMSO) or 50 mM AS1 was infused into agarose lining one side of a square petri dish. As shown, larval zebrafish are not drawn towards the AS1 source. N = 27 larvae in the DMSO group, 16 in the AS1 group. B Distance of each individual larva from the AITC stimulus at the final time point (840 s) for the experiment shown in A. C Chemical attraction/aversion assay in which 2 mL of 1% DMSO or variable concentrations of AS1 (in 1% DMSO vehicle). Larval zebrafish do not appear to swim towards the AS1 source at any concentration tested for the duration of the assay. N = 36 fish for the DMSO condition, 36 for the 10 μM AS1 condition, 36 for the 25 μM AS1 condition, 40 for the 50 μM AS1 condition, and 40 for the 100 μM AS1 condition. D Same as C, but with 10% DMSO or 1 mM AS1 (in 10% DMSO). N = 36 larvae in both conditions. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-tailed unpaired t test used in B. Two-way ANOVA with Sidak’s multiple comparisons test used in A, D. Two-way ANOVA with Tukey’s multiple comparisons test used in C