Fig. 5

Analysis of stress-induced gene expression changes. A Volcano plot depicting differential expression results, comparing worms that experienced starvation (str) to worms that grew under normal conditions. Each dot represents a gene. Differentially expressed genes with log₂ (fold change) ≥ 1 were painted in red, and differentially expressed genes with log₂ (fold change) ≤  − 1 were painted in blue. B A proportional Venn Diagram depicting the intersections between genes that are significantly downregulated under heat shock, osmotic stress, or starvation, compared to their matching control samples. C Log₂ (Fold enrichment) score for a curated list of epigenetic genes, in the set of genes significantly downregulated under all stress conditions. The p-value for enrichment was calculated using 10,000 random gene sets identical in size to the tested group. *** indicates adj. p-value ≤ 0.001