Fig. 5
From: 1,6-Hexanediol regulates angiogenesis via suppression of cyclin A1-mediated endothelial function
1,6-Hexanediol affects endothelial cell cycle. A, B HUVECs were treated by two doses of 1,6-HD. HD lower: 1 mg/ml, HD higher: 3.3 mg/ml. Cells were sent to Beijing Genomic Institution (BGI) for RNA sequencing analysis. The most downregulated genes in 1,6-HD treated cells were analyzed by online gene set enrichment analysis (GSEA) program. The most changed top 10 cell cycle genes with dose-dependent manner after 1,6-HD treatment were listed. C HUVECs were treated with 1,6-HD at indicated concentrations. Cell cycle was analyzed by flow cytometry. D–F The percentages of S phase, G1 phase, and G2/M phase were further analyzed. Mean viability was shown and standard error of the mean presented the standard deviations of duplicated samples. *p < 0.05, **p < 0.01