Fig. 6
From: 1,6-Hexanediol regulates angiogenesis via suppression of cyclin A1-mediated endothelial function
1,6-Hexanediol suppresses the expression of cyclin A1. A Western blot analysis of the G1/S transit-related genes in HUVECs treated with or without 1,6-HD. GAPDH was used as the loading control. B The mRNA expression of CCNA1 at different concentrations of 1,6-HD treatment was determined by qRT-PCR. C HUVECs were treated with 1,6-HD at indicated time points. Cells were collected and the expression of cyclin A1 was analyzed by western blot. D In the parallel experiment, the mRNA expression of CCNA1 was determined by qRT-PCR. E The expression of cyclin A1 was examined in Matrigel plugs containing 1,6-HD by Western blot. F The mRNA expression of CCNA1 in cornea was determined by qRT-PCR. G, H HUVECs were treated with bortezomib or MG132 for 1 h and then treated by 1,6-HD at indicated times. The expression of cyclin A1 was analyzed by Western blot. GAPDH was used as the loading control. Mean viability was shown and standard error of the mean presented the standard deviations of triplicate samples. *p < 0.05, **p < 0.01, ***p < 0.001