Fig. 7
From: 1,6-Hexanediol regulates angiogenesis via suppression of cyclin A1-mediated endothelial function
1,6-Hexanediol suppresses endothelial function through cyclin A1. A HUVECs were infected with lentiviral particles encoding CCNA1 open reading frame. After 36 h, the infected cells were treated with indicated 1,6-HD for 24 h. Tube formation assay was carried out following. B Quantification analysis of A. C Cell proliferation was examined by CCK8 assay in HUVECs overexpressed by CCNA1. D, E The overexpression of Cyclin A1 was analyzed. F The expression of endogenous cyclin A1 and exogenous Flag-cyclin A1 was detected. Mean viability was shown and standard error of the mean presented the standard deviations of triplicate samples. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar: 200 μm