Fig. 7

Multiple staining rounds with hCLARITY. (top row) Stained sections from case p1 mounted in SlowFade were de-stained in clearing solution for 24 h at 60 °C (left). A de-stained section was mounted and imaged as control (middle). Re-staining with cellular markers (right). Scale bars, 2 µm (left and middle), 10 µm (right). (bottom row) DAPI channel of the images shown in the top row before de-staining (left), after de-staining (middle), and after re-staining (right). Imaging parameters were identical. Scale bars, 10 µm