Fig. 1
From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
Drosophila FOI is required for larval fat body development. A Morphological analysis indicated a decrease in larvae size and a significant fat body developmental defect of foi RNAi third instar larvae, whereas no significant difference was observed for foi OE. The green line marks present the fat body tissues. n = 6 replicates per group. B Morphological analysis indicated a decrease in pupal size of foi RNAi compared with control, whereas no significant difference was observed for foi OE. n = 10 replicates per group. C Quantitative measurement of (B) n = 10 replicates per group. D Eclosion rate of Cg-Gal4 > foi RNAi was reduced, whereas no significant difference was observed for Cg-Gal4 > foi OE. n = 50–70 larvae per vial, n = 6 vials per experimental group. E Knockdown of foi led to reduced fat body size and cell size in the fat body, whereas no significant difference was observed for Cg-Gal4 > foi OE. n = 6 replicates per group. Scale bar, 100 μm. F Quantitative measurement of the fat body cell sizes in (E). n = 6 replicates per group. Genotypes used in (A-F) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi or Cg-Gal4 > foi OE. All values are presented as mean ± SEM of the biological replicates. **p < 0.01, ***p < 0.001; two-tailed Student’s t-test. OE, overexpression