Fig. 2
From: Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility
Phenotype analysis of Ck137956 knockout male mice. A HE-stained sections of the caput and cauda epididymis from Ck137956+/- and Ck137956−/− mice. Scale bars: 50 μm. B HE-stained sections of the testes from Ck137956+/- and Ck137956−/− mice. PAC, pachytene spermatocytes; RS, round spermatids; ES, elongated spermatids. Scale bars: 50 μm. C Immunofluorescence analysis of seminiferous tubules from Ck137956+/- and Ck137956−/− testes using PNA (red) stained the acrosome and DAPI (blue) stained the nuclei. Magnification of round spermatids were shown in white. Scale bars: 50 μm. D Ultrastructural analysis of Ck137956+/- and Ck137956−/− round spermatids by transmission electron microscopy. The developmental stage of round spermatids was determined by acrosomal angles. Nu, nucleus; Acr, acrosome. Scale bar: 1 μm. E Immunofluorescence staining of SYCP3 (green) and γH2AX (red) on spread nuclei of spermatocytes from Ck137956+/- and Ck137956−/− testis. Scale bar: 5 μm. n = 3. Data were presented as the mean ± SEM. NS, not significant. F TUNEL staining (green) and quantitative analysis of Ck137956+/- and Ck137956.−/− seminiferous tubules. DAPI (blue) stained the nuclei. Asterisks indicated TUNEL-positive tubules. Scale bar: 50 μm. n = 3. Data were presented as the mean ± SEM. **p < 0.01