Fig. 5
From: Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility
Ck137956 alters the mRNA-stabilizing ability of Miwi. A Immunofluorescence analysis of LINE1 (green) in Ck137956+/-, Ck137956−/− and Pnldc1−/− testes. DAPI (white) stained the nuclei. Scale bars: 50 μm. B qRT-PCR analysis of the mRNA expression levels of Ppp1cb, Atr, Tox4, Gfpt1, Psmag, Mdc1 and BC026590 in Ck137956−/− and control round spermatids. n = 3. C Distribution of Ck137956 and Miwi proteins in different fractions collected from sucrose gradient sedimentation of testis lysate. Rps3 and Msy2 were used as mono/polysomes and mRNPs controls, respectively. D qRT-PCR analysis of Tssk1, Tssk2, Tnp1, Tnp2, Prm2 mRNA expression levels in mRNPs of Ck137956+/- and Ck137956−/− testes. n = 9. E qRT-PCR analysis of mRNAs expression levels in GC-2spd(ts) cells treated with siCk137956 or/and siMiwi with scrambled sequence as a control. DK, doubleknock down group. The statistical significance between the groups was plotted. n = 3. F Quantitation of mRNA stability in siMiwi-treated and control GC-2spd(ts) cells after Actinomycin D treatment (10 μg/ml). The upper right corner shows the half-life time (t1/2) of gene expression in siMiwi-treated and control cells. n = 3. G qRT-PCR analysis of mRNA expression in siCk137956-treated cells with or without Miwi overexpression. n = 3. H qRT-PCR analysis of mRNA expression levels in siMiwi-treated cells with or without Ck137956 overexpression. n = 3. All data were represented as mean ± SEM. NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001