Fig. 6

Analysis of candidate pigmentation genes that may act downstream of Aristaless1. A The top panel highlights distinct sections of the adult wing analyzed (left) and a pathway model (right) for the candidate genes of interest. The model showcases a view of the scale and socket cells and highlights the genes involved in the synthesis and transport (direct or after synthesis) of 3-hydroxykynurenine (3-OHK) yellow pigment. Enzymes: Kynurenine formamidase (Kf), Cinnabar (Cb); Transporters: White (Wt), Scarlet (Sc), Karmoisin (Kar). B Relative expression levels of each candidate gene in white and yellow pupal forewings sections (proximal, media, distal) across 3 different time points (4, 6, and 7 days APF). The relative expression values are scaled to the highest value across the wing sections for each of the genes. Enzymes are shown in the middle panel and transporters on the bottom panel. Statistical significance was assessed using t-test and p values are shown for significant (asterisk) and nearly significant comparisons