Fig. 2

Immunodetection of Al1 and Al2 in early (36–42 h after deposition) wild type and –Al1 CRISPR Heliconius cydno embryos. A Immunodetection of Al1 and Al2 in a wild-type embryo (lateral-view). B Al1 in situ hybridization. C Immunodetection of Al1 and Al2 in a wild-type embryo (ventral view). D Immunodetection of Al1 in an -Al1 CRISPR embryo (data from [3]). White brackets highlight the different anatomical sections in A and B. The segments are also labeled on the merge images (Thoracic [T], Abdominal [A]). Panels show detection of DNA (A′, C′, D), Al1 (A″, C″, D″), Al2 (A″′, C″′, D″), and a merge (A, C, D″). 250-μm scale bars are shown on all merged images