Fig. 4
Detailed comparison of the immunodetection of Al1 and Al2 between early (36Â h after deposition) and late (48Â h after deposition) wild-type eyes of Heliconius cydno embryos. A Immunodetection of Al1 and Al2 in early wild-type embryonic eyes. B Immunodetection of Al1 and Al2 in late wild-type embryonic eyes. C Deeper z-plane view of the B panel showcases the detection of Al2 within the cell bodies projecting into the brain. D Caterpillar eye following eclosion. Panels show merged views (A, B, C). The insets of the merged images highlight DNA, Al1, and Al2 detection. Scale bars are shown on the merged images and Panel D