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Fig. 3 | BMC Biology

Fig. 3

From: Massive expansion of sex-specific SNPs, transposon-related elements, and neocentromere formation shape the young W-chromosome from the mosquitofish Gambusia affinis

Fig. 3

A Comparative expressed sequence hybridization (CESH) of differentially labeled testis and ovary cDNA libraries demonstrates expression of repetitive elements from both sexes enriched along the entire Wq (arrowhead). In addition, a medium-sized acrocentric pair in females (left) and males (right) showed a male-expressed repeat cluster in the long-arm subtelomeric region (asterisks). B Immuno-fluorescence staining of hypermethylated DNA using an anti-5mC antibody showed no enrichment of DNA methylation of the W-chromosome (arrowhead) indicating that the Wq region does not comprise transcriptionally silenced chromatin. Intriguingly, in both female (left) and in male (right) metaphases, a presumably homologous chromosome pair of autosomes was entirely hypermethylated in all analyzed mitoses (asterisks)

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BMC Biology

ISSN: 1741-7007

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