Fig. 5

A–D FISH mapping of fluorescent PCR products from exonic and intronic regions of the amt locus to female and male G. affinis metaphases. E Summary of the results together with the Ensembl map detailing exon/intron structure of the gene. Green bars indicate PCR probes B, D, G, H, M, R, P, and hAT, yielding FISH signals on Gambusia Wq. Red bars correspond to PCR probes A, C, E, F, O, and TC1 without FISH signals on Wq. F Synteny map of W and Z with the location of the amt gene on Wq (amtW) and on Z (amtZ), each shown by a red dot. Green dots indicate TE-related repeats with partial homology to hAT, Helitron, and MuDR that were returned from a BLAST search, using the hAT-related sequence from the amt locus as input, and that were also identified by FISH using PCR products D and hAT. Both sequence analysis and FISH showed high enrichment of these repeats on the long arm of W, and absence on Z