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Table 1 Chosen candidates for antibody production

From: A small protein coded within the mitochondrial canonical gene nd4 regulates mitochondrial bioenergetics

Approach used

Amino acid sequence (antigenic sequence in bold underlined)

Length

Predicted MW

Position in the reference human mtDNA

OpenProt IP_306387

IFYLSRPRNKHASFYSSSNQKNKPSFHRSCHQVFPHASNRIHNPSNSYPLQQYTLRTMNHNQYYQSMLIINNHNSYSNKTRNSPLSL

LSPRGYPRHPSDIRPASSH MTKTSPHLNHMPNLSLTKRKPSPHSLNLIHHSRQLRWIKPNPATQNLSMLLNYPHRMNNSSSTVQPa

172aa

20 kDab

Within nd2 sense, frameshift 4547 to 5063

OpenProt IP_306389

ICNNLLHSNTHHNRRLWQLTSSPNNRCPRYGVSPHKQHKLLTLTSLSPTPARICYSGGRSRNRLNSLPSLSRELLPPWSLRRPNHLLL

TPSRCLLYLRGHQFHHNNYQYKTPCHNPMPNAPLRLIRPNHSSPTSPISPSPSCWHHYTTNRPQPQHHLLRPRRRRRPHSMPTPILI FRSPWSLYSYPTRLRNNLPYCNLLLRKKRTIWMHRYGLSYDINWLPRVYRVSTPYIYSRNRRRHTSMFHLRYHNHRYPHRRQSIa

259aa

31.2 kDad

Within cox1 sense, frameshift 6089 to 6866

OpenProt IP_306398

MSSSKPHLSPPWLSSPDEATSQNAWTQAHTSYSTPa

35aa

3.8 kDac

Within nd4 sense, frameshift 11,115 to 11,219

OpenProt IP_306403

MAESSATFTPMAPQYSLSASSYTSGEAYITDHFSTQKPETSALSSCLQL a

49aa

5.2 kDac

Within cytb sense, frameshift 14,970 to 15,116

MS/MS

MGLSRIEGLFGQVVCGGLGMCFLVLHRAIIGMWLVCWLVGLVWGALWSGSEITWLGRRSLGGLRGPLLGVMGWVLLYDRHVIGGSLCVVVQVEAY

95aa

10,3kDac

cox3 anti-sense 9462 to 9177

MS/MS

MWSLPRRLPGWPSSARMRRLRAVPRTPAHAPNNRYSVPMSLWFVENSQRSANISGGEVKWLSEALDCKSKDRG

73aa

8,3kDac

cox1 anti-sense 6061 to 5842

MS/MS

IMRMTAPVKLQGVWMRMAVTTRAMWLIEEYAMSDFRSVCRRQMELVMIMPHRDSTRKG

57aa

6,9kDab

nd4 anti-sense 11,709 to 11,535

MS/MS

MRHNYNKLHLPTTNRPKIAHCMLFNQPHSPRSNSHSHPNPLKLHRRSHSHNRPRAYILITILPSKLKLRTHSQSHHNPLSRTSNSTPTNSFLMTSSKPR

99aa

11,5kDae

Within nd4 sense, frameshift 11,557 to 11,854

  1. aThe sequence is presented with AGA and AGG codons translated to arginine, and the sequence in OpenProt is underlined (OpenProt considers AGA and AGG as stop codons for the mitogenome)
  2. bUndetected by western blot
  3. cMultiple bands > 40 kDa detected by western blot
  4. dWestern blot signal of expected weight, but that did not disappear in Rho0 cells devoid of endogenous mtDNA (ethidium bromide-treated HeLa-ρ0 obtained from Abcam [ab154479]), which renders improbable its mitochondrial origin
  5. eMTALND4