Fig. 7

Chaperone gene expression during oral siphon regeneration. A qRT-PCR analysis of hsp70, dnaJb4, and bag3 transcript levels at day 3 after oral siphon (OS) amputation compared to day 0 controls. Asterisks: significance (p = .000087, p < .00001, and p = .001156 from left to right) by one-tailed Student’s t test with ∆Ct. B–M hsp70 (B–E), dnaJb4 (F–I), and bag3 (J–M) unoperated controls at day 0 (C, G, K) and regenerating animals at day 3 (B, D, F, H, J, L), and day 5 (E, I, M) after OS amputation. B, F, J Entire animals. C–E, G–I, and K–M Magnified regions of the branchial vasculature (arrowheads in B, F, I). The digestive tract (DT) is unstained but appears dark due to the presence of food and feces. NC, neural complex; AS, atrial siphon; EN, endostyle. Scale bar in B: 50 μm; same magnification in B, F, J. Scale bar in C: 15 μm; same magnification in C–E, G–I, and K–M. Data from three replicate experiments