Fig. 8
Effects of siRNA knockdown on chaperone gene expression and oral siphon regeneration. A–F In situ hybridization showing hsp70 (A, B), dnaJb4 (C, D), and bag3 (E, F) expression in the branchial sac vasculature (arrowheads) in control siRNA (A–E), hsp70 siRNA (B), dnaJb4 siRNA (D), and bag3 siRNA (F) treated animals at day 5 after OS amputation. Scale bar: 20 μM; same magnification in A–F. G–K Effects of siRNA gene knockdown on oral siphon (OS) regeneration. G Percent regeneration in control, hsp70, dnaJb4, and bag3 siRNA-treated animals at day 5 after OS amputation. The number of animals is shown at the base of each bar. Asterisk: significance (χ2 = 53.23, p < .000001) by chi-square test. H–K Distal edges of the OS at day 5 after OS amputation in control siRNA (H), hsp70 siRNA (I), dnaJb4 siRNA (J), and bag3 (K) siRNA-treated animals. OPO, oral siphon pigment organ; CMB, circular muscle bands. Combined data from 5 replicate experiments