Fig. 9

Effects of siRNA knockdown on EdU labeling in the branchial sac and the amputated oral siphon. A The EdU pulse-chase labeling regime used in these experiments. OS amputation occurred at day 0 and was followed immediately by siRNA and EdU incubation, and EdU was chased and fresh siRNA added at day 2. B–H EdU labeling in the branchial sac vasculature (B–E) and oral siphon (OS) (B, F–H) in control siRNA (C, F), hsp70 siRNA (D, G), and dnaJb4 siRNA (E, H) labeled animals at day 5 after OS amputation and EdU pulse-chase labeling. CMB, circular muscle bands; SCN, branchial sac vasculature. Scale bar: 10 μm: same magnification in C–E and F–H. See Fig. 1A for location of EdU quantification areas. EdU labeling of 10 animals was determined for each bar. NS, no significance. Asterisk: significance (f = 52.32, df = 27, p < .00001) by one-way ANOVA with post hoc Tukey HSD. Combined data from three replicate experiments