Fig. 3

The dopaminergic system in the mouse retina during development. A Flatmount retinas showing tyrosine hydroxylase (TH) immunopositive cells in wild-type retinal explants that were cultured for 2-DIV at P8 and P30. Scale bar = 20 µm. B Schematic representation of retina sampling at the trough and the peak of PER2::Luc oscillations after 2-DIV (left). Ex vivo (CT0 and CT12) and in vitro relative expression of tyrosine hydroxylase (Th) mRNA at P8 (right). C Means of the endogenous period, the phase and the amplitude of the retinal clock at 3 postnatal developmental stages (P8, P15 and P30). Retinal explants were supplemented with DA (50 µM), Apo (50 µM), or a combination of Res + L-AMPT (respectively 10 µM and 100 µM) and are compared to non-supplemented control retinas. The total numbers of retinas used were: P8: C, n = 10; DA, n = 7; Apo, n = 4; Res + L-AMPT, n = 8); P15: C, n = 8. DA, n = 6; Apo, n = 3; Res + L-AMPT, n = 5; P30: C, n = 8; DA, n = 9; Apo, n = 5; Res + L-AMPT, n = 6. Bars represent the mean ± SEM (ANOVA, * = p ≤ 0.05; ** = p ≤ 0.01)