Fig. 6

The blockade of acetylcholinergic waves shortens the period of the retinal clock. A Means of the period and the phase in controls (C) and retinas supplemented with mecamylamine acid (MMA) in wild-type and Opn4 ^−/−::Per2 ^luc retinas at P8. The total numbers of retinas used were: WT: C, n = 10: MMA, n = 6; Opn4 ^−/−: C, n = 11; MMA, n = 4. Data from WT and Opn4 ^−/− ::Per2 ^Luc control retinas are already presented in Fig. 1C. Bars represent mean ± SEM (ANOVA, * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001). B Mean light-induced phase shift after a 465 nm light stimulation at CT16 (30 min, 10¹⁴ photons/cm²/s) in wild-type and Opn4^−/−::Per2 ^luc retinas with or without MMA supplementation (MMA). Bars represent mean ± SEM (C: n = 4 for both genotypes; MMA, n = 4). Statistical differences with the C are indicated by **: p ≤ 0.01; ***: p ≤ 0.001