Fig. 5
From: Costs of reproduction are present but latent in eusocial bumblebee queens
Comparison of changes in mRNA-seq gene expression profiles with relative age in Bombus terrestris queens. Euler diagrams of overlaps between differentially expressed genes (DEGs), i.e. genes differentially expressed between the two time-points TP1 and TP2 within treatments and shared between R queens (eggs removed) and C queens (eggs removed and replaced) for: a, b brain; c, d fat body; and e, f ovaries. (In panel e, because their low values mean there is a lack of space, numbers of DEGs for R queens and shared between R and C queens are shown adjacent to the relevant area.) Asterisks (*), significant overlap in DEGs (Fisher’s exact test, p < 0.05 after Bonferroni correction). Upregulated DEGs: DEGs significantly more expressed in TP2G than TP1G, i.e. that increase expression with queen relative age; downregulated DEGs: DEGs significantly more expressed in TP1G than TP2G, i.e. that decrease expression with queen relative age. Results of statistical tests are in Additional file 2: Table S7 and identities of overlapping genes are in Additional file 2: Table S8. Sample sizes: brain: R:TP1G (N = 6), R:TP2G (N = 5); C:TP1G (N = 6), C:TP2G (N = 5); fat body: R:TP1G (N = 6), R:TP2G (N = 6); C:TP1G (N = 6), C:TP2G (N = 4); ovaries: R:TP1G (N = 6), R:TP2G (N = 6); C:TP1G (N = 6), C:TP2G (N = 6)