Fig. 4

ML phylogenetic tree of Leishbuviridae based on RDRP amino acid sequences. Numbers at the branches indicate Bayesian posterior probability (PP) and ML bootstrap supports (BS). Only supports PP ≥ 0.8 and BS ≥ 50 are shown, lower values are replaced with dashes. Circles correspond to maximal statistical support by both methods. The scale bar indicates the number of substitutions per site. The double-crossed branch is at 50% of the original length. Outgroup is shown in gray. Inversed font and background colors indicate the strains studied in this work. Single asterisk marks viruses detected in trypanosomatid cultures, and two asterisks indicate viruses found in metatranscriptomes along with trypanosomatids (detected previously in [14] or reported in a corresponding NCBI Sequence Read Archive record in the “Taxonomy Analysis” section)