C. glutamicum strain | Locus tag | Protein/enzyme | His-Titer [mM] | His-Titer [%] | Biomass [final OD600] |
---|---|---|---|---|---|
CgHis2 reference |  |  | 11,5 ± 0.6 | - | 19.3 ± 0.5 |
CgHis2 ΔfasB | NCgl2409 | Fatty acid synthase | 14.3 ± 0.4 |  + 25 | 18.4 ± 0.7 |
CgHis2 ΔNCgl2964 | NCgl2964 | DEAD/DEAH box helicase | 10.6 ± 0.3 |  − 8 | 18.5 ± 0.2 |
CgHis2 ΔfasA | NCgl0802 | Fatty acid synthase | No deletion possible |  | |
CgHis2 Δpks | NCgl2773 | Type 1 polyketide synthase | No deletion possible |  | |
CgHis2 Δemb | NCgl0184 | Arabinosyl transferase C | No transformation of pSenHis[hisEG] possible |  | |
CgHis2 ΔgltB | NCgl0181 | Alpha subunit of glutamate synthase | 11.6 ± 0.2 | 0 | 21.9 ± 0.1 |
CgHis2 ΔNCgl0552 | NCgl0552 | DNA segregation ATPase | 11.1 ± 0.6 |  − 4 | 19.6 ± 0.6 |
CgHis2 Δcps | NCgl2618 | Non-ribosomal peptide synthase | 13.6 ± 0.2 |  + 18 | 18.6 ± 2.3 |
CgHis2 ΔpknB | NCgl0040 | Eukaryotic-type serine/threonine kinase | No deletion possible |  | |
CgHis2 ΔNCgl2959 | NCgl2959 | Phosphoesterase | 10.9 ± 0.2 |  − 6 | 19.4 ± 0.4 |
CgHis2 ΔNCgl1737 | NCgl1737 | Hypothetical membrane protein | 10.8 ± 0.3 |  − 8 | 18.8 ± 0.2 |
CgHis2 ΔNCgl2981 | NCgl2981 | Hypothetical protein | No deletion possible |  | |
CgHis2 ΔggtB | NCgl0916 | Putative gamma-glutamyltranspeptidase precursor PR | 10.3 ± 0.4 |  − 12 | 20.0 ± 0.4 |
CgHis2 ΔNCgl0705 | NCgl0705 | Probable ATP-dependent helicase | 11.8 ± 0.5 |  + 2 | 18.8 ± 0.5 |
CgHis2 ΔputA | NCgl0098 | Proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase | 9.7 ± 0.4 |  − 17 | 20.3 ± 0.5 |
CgHis2 Δpyk2 | NCgl2809 | Pyruvate kinase | 10.6 ± 0.3 |  − 8 | 18.1 ± 0.4 |
CgHis2 Δpyk1 | NCgl2008 | Pyruvate kinase | 12.1 ± 0.4 |  + 5 | 21.5 ± 1.0 |