Fig. 2

Heat map of SHANK2 expression in analyzed human and mouse brain regions (neuropil). The neuropil sum intensities (mean) of Additional file 4a,d are represented in the sketches, i.e., color intensities represent SHANK2 expression in the neuropil of each subregion. The applied heat map is shown at the bottom (intervals of 0.01, CB was dyed with the color intensity corresponding to 1). For the CTX and THA (mouse), the color from the analyzed subregion was extrapolated to the entire region since no strong differences were seen in the overview scans. The analyzed subregions are dashed. For the HC (mouse), intensities for all layers were determined by comparing the analyzed region(s) to the non-analyzed layers in overview scans. Overall, crossed out regions were not analyzed; gray regions were only analyzed in the other species. The sagittal sections are based on “Sobotta, Atlas der Anatomie; Kopf, Hals und Neuroanatomie” (24^th edition, p. 271) and the used mouse brain atlas (see “Methods”). The coronal sections are based on “The Atlas of the Human Brain.” 1 = CD, 2 = PUT, 3 = EGP, 4 = IGP, 5 = AMY (mouse: to present all regions, the extended AMY was colored as substitute), 6 = SN, 7 = HT, 8 = HC, 9 = STH (see Table S1)