Fig. 3

SHANK2 expression in the human hippocampus, medulla oblongata, and striatum. a SHANK2 staining (Sigma antibody) in a human hippocampus, representative for all cases. Representative stainings are shown as inverted (inv.) image (white = no signal). The dashed region is shown in the middle as merge image of SHANK2/VGLUT1/DAPI IF, high magnified insets of three layers are depicted on the right. Scale bars, 500 µm (left), 100 µm (middle), 5 µm (right). b SHANK2 distribution in a mouse hippocampus as revealed by two different antibodies. Staining is depicted in the same manner as in a (left). Scale bar, 300 µm. c SHANK2 IF (inv.) in a human medulla oblongata section (right), opposed to pigment Nissl staining (left). Scale bars, 1 mm. Insets of selected regions are shown on the right. Scale bar, 20 µm. d Vessel-related islands of enriched SHANK2 IF (*) in the human striatum. Scale bar, 1 mm (top). A CD section was stained for TH, de-stained, and re-stained for SHANK2 (bottom). Scale bar, 300 µm. ic = internal capsule