Fig. 2

Synaptic transmission defects are corrected by flailer gene editing. A Representative traces of miniature AMPA receptor currents from wild-type, FL, and FL1 infected neurons. Quantification of the amplitude (B) and frequency (C) of currents. D Representative traces of current clamp recordings showing spiking of neurons. E Spike frequency of wild-type, FL, and FL1 neurons at 4, 6, or 8 days after infection. For A–E At least 20 neurons for each condition from 3 independent neuronal cultures were analyzed. F Calcium imaging using GCamp6 at 4–6–8 days after infection. A total of 500 neurons from at least 3 independent neuronal cultures were analyzed. G LTD induction by PP-LFS in hippocampal slices of wild-type, FL, and FL1 animals at P22-23 after HSV infection. 9 slices from 3 different animals were used for each condition. Bars represent mean ± SEM; ***p < 0.001. One-way ANOVA was used to determine significance compared to wild-type condition