Fig. 3

Injection of FL1 AAV into the ventral hippocampus results in highly efficient flailer gene editing. A Representative image of ventral hippocampus stereotactic injection showing a high infection rate by TdTomato signal at the targeted region. Infected ventral hippocampus tissue was extracted to determine Protein (B) and mRNA (C) expression levels from wild-type, FL, and FL1-injected animals. mRNA levels are relative to FL animals and GAPDH was used to normalize. D–F Single nuclei PCR to determine gene editing in FL1icv injected Flailer animals. D Scheme depicting the expected PCR products of FL animals (~ 1200 bp), FL edited heterozygous (~ 1200 bp +  ~ 500 bp), and FL edited homozygous (~ 500 bp). E Representative gel of PCR products from single nuclei FL1 ventral hippocampus infected tissue. F Quantification of the number of FL, FL heterozygous, and FL homozygous products. A total number of 500 cells were analyzed from 10 FL1 ventral hippocampus-injected animals