Fig. 5

FL1icv AAV injection results in highly efficient flailer gene editing. A Representative image of intracerebroventricular injection showing high and extensively distributed infection by TdTomato signal. B Magnification of the hippocampus area is depicted in a yellow square in A. Protein (C) and mRNA (D) expression levels of infected tissue extracted from the cortex of wild-type, FL, and FL1icv-injected animals. E Scheme depicting the expected PCR products of FL animals (~ 1200 bp), FL edited heterozygous (~ 1200 bp +  ~ 500 bp), and FL edited homozygous (~ 500 bp). F Representative gel of PCR products from single nuclei FL1icvl infected cortical tissue. G Quantification of the number of FL, FL heterozygous, and FL homozygous products. A total number of 500 cells were analyzed from 10 FL1icv animals. Bars represent mean ± SEM; ***p<0.001. One-way ANOVE was used to determine significance compared to FL condition