Fig. 5

DSL could compensate for the missing cluster insertions. A Example of a DSL in Canton-S. Note the typical signature of DSL where antisense piRNAs (red, negative y-axis) align upstream of the TE insertions (F-element) and sense piRNAs (blue, positive y-axis) downstream of the TE [31]. B Abundance of DSL in the five investigated strains (dark gray). As a negative control we also computed the fraction of BUSCO genes with a typical DSL signature (light gray; BUSCO genes should not have DSL signatures). C Abundance of piRNA producing loci in the five strains. Data are shown for cluster insertions (red) as well as cluster insertions plus DSL (cyan). Note that the number of families without piRNA producing locus is dramatically reduced when DSL are considered