Table 1 Sequences of primers for real-time PCR
From: Lactoferrin deficiency during lactation increases the risk of depressive-like behavior in adult mice
Gene name | F-primer (5’-3’) | R-primer (5’-3’) |
|---|---|---|
Gapdh | TCTCCTGCGACTTCAACA | TGTAGCCGTATT CATTGTCA |
Zo-1 | GCATCATTCGCCTTCATAC | GACACAACCTCATCCTCAT |
Occludin | GGCTGCTGCTGATGAATA | ATCCTCTTGATGTGCGATAA |
TLR4 | CACTGTTCTTCTCCTGCCTGA | GGAATGTCATCAGGGACTTTGC |
LBP | GTCGTGGGCAGTACGAGTTT | CCTTCCATTTGCCTCGGACA |
MyD88 | CGCATGGTGGTGGTTGTTTC | AGTCGCTTCTGTTGGACACC |
CD14 | ACAATTCACTGCGGGATGCT | AGCTCATCTGGGCTAGGGTT |
JNK | CCACCAAAGATCCCGGACAA | GGCTGCCCTCTTATGACTCC |
NFκB | CCCTACGGAACTGGGCAAAT | GCAAATTTTGACCTGTGGGT |
IκBα | CCTGACCTGGTTTCGCTCTT | CTGTATCCGGGTACTTGGGC |
P38MAPK | TTCTACCGGCAGGAGCTGAA | ATCAAAAGCAGCACACACCG |
IL-1β | CTTCAGGCAGGCAGTATC | CAGCAGGTTATCATCATCATC |
TNF-α | ACTGAACTTCGGGGTGATCG | CCACTTGGTGGTTTGTGAGTG |
Bdnf | TAAACGTCCACGGACAAGGC | AGTGTCAGCCAGTGATGTCG |
Grb2 | AACATCCGTGTCCAGGAACC | AAGTCTCCTCTGCGAAAGCC |
CaMK1 | TGATCCTGGCAGAGGACAAGA | GCTACAATGTTGGGGTGCTTG |
Creb | AACCAGCAGAGTGGAGATGC | GATGTTGCATGAGCTGCTGG |
Lrg1 | TGTCCAGCCTCAAGGAATGC | TTCCACCGACAGATGGACAG |
Gbp4 | TGAACCAGGAAGCCATAGAGA | GGGAAACCTTTGGCTGGTAGG |
Stx11 | ATGACTTTGACGCTCCTCGG | GCGCCTCACGTCTATCAGAA |
Pigr | GACTCTCGCTGGAGAACCAC | ACGGATAGTGGCAGGAAACG |
Ltf | AGCTGAAGTCTACGGGACCA | CTCAGGCCTTGGAGTTGGTT |
Lcn2 | ACAACCAGTTCGCCATGGTAT | AAGCGGGTGAAACGTTCCTT |
S100a9 | ACCACCATCATCGACACCTTC | AAAGGTTGCCAACTGTGCTTC |
Ccl28 | CTCACACTCATGGCTGTGGCT | AGTACGATTGTGCGGGCTGA |
IL-25 | GACCTGTACCACGCTCGATG | AGAAGACCGTCTGGTTGTGG |
Ccn5 | TGTGTGACCAGGCAGTGATG | GTGCTCCAGTTTGGACAGGG |
Cdh3 | GCATGCACCATGCAGACAAT | GTGGCATCACCCACTCTCTC |
Prdm12 | TCAAGTGTGCCCGGAATGAG | TCCTGGTCTGGAGGGATCAT |
Pcdha8 | TCAAAGGGTTTTGGGGACCT | GAGCCTTTTGTGTCGCTGGA |
Trpv4 | TGGAACCAGAACTTGGGCAT | GGACCAACGATCCCTACGAA |
Gabra6 | AGGAGTCAGTCCCAGCAAGA | ATGAACCAATCCATGGCGGT |
Kcne2 | CCAGAGTGGATGCCGAGAAC | CTTCGACTTCACCGTGCTCA |
Kcnj13 | TTTGTGGCGAAGATTGCACG | ACACGAACGTTGGTCAGAGG |