Fig. 1

Reproducibility of transcriptome-wide eIF4A3 eCLIP-seq peaks in human cells. a The positioning of 5′ ends of human eIF4A3 eCLIP reads relative to the exon junction for two eCLIP replicates (in blue) and two SMInputs replicates (in gray). The truncation signal is normalized by both exon distribution and library size. The eCLIP signal peaks at a position 27 nucleotides (nt) upstream of exon-exon junctions. b A Venn diagram illustrating eIF4A3 peaks detected by PureCLIP in eCLIP replicates 1 and 2. The number of peaks in common or not between the two replicates is indicated. The peak agreement between the two replicates corresponds to the calculated Jaccard index (JI). c A barplot showing the distribution of Jaccard index values for peaks detected using PureCLIP analysis of replicates of eCLIP datasets for 84 different RBPs from ENCODE. The median value of the distribution is 0.2 (dotted line)