Fig. 4

Combination of eCLIP and ipaRt data analysis establishes the universal EJC loading. a Distribution of the middle position of ipaRt reads relative to the exon junction for three replicates (in blue) and two control mRNA-seq (in gray). The signal peaks at a position 23 nucleotides (nt) upstream of exon-exon junctions. b A bar plot illustrating detected exons by ipaRt in 10 different expression classes (RPKM class interval). Classes were created with equal bin sizes and for exons from all genes excluding the last exons. The percentage of loaded exons is indicated on top. c A bar plot showing, among all commonly detected genes, the number of exons detected as loaded in both experiments (in dark blue), unloaded in both experiments (in yellow), and variably detected between conditions (in light blue) within each expression class. d A bar plot representing detected exons in at least one of the datasets in different expression classes with equal bin sizes for exons from all genes excluding the last exon. The percentage of loaded exons is indicated on top