Fig. 3

X-ray irradiation disrupts the development of ovary. Representative confocal microscopy images of ovaries (A–H) and ovarioles (I–P) with Vasa (Green), Phalloidin (Red), and DNA (Grey) staining at day 0 (A and I), day 1 (B and J), and day 3 (C and K) in control females, and day 0 (D and L), day 1 (E and M), day 3 (F and N), day 7 (G and O), and day 14 (H and P) in irradiated females after irradiation. A–H Red arrows indicate one ovariole (green marked by Vasa staining) in each sample. I,J,L and M White arrows indicate the future oocyte as indicated by the condensed chromosome. Yellow arrows in J and K indicate follicular cells bisecting the germarium into two segments, and the yellow arrow in M shows that no follicular cells bisect the germarial region of the ovariole from irradiated females. K, N-P follicular cells are present in control follicles (yellow arrowhead in K) but absent in ovarioles from irradiated females (yellow arrowheands in N–P). Scale bars in A (for A–H) and I (for I–P) are 50 µm