Fig. 4

X-ray irradiation blocks ovariole maturation. A–J Representative confocal microscopy images of ovarioles with Vasa (Green), PH3 (Red), and DNA (Grey) staining at day 0 (A), day 1 (B), day 3 (C), day 7 (D), and day 14 (E) in controls, and at day 0 (F), day 1 (G), day 3 (H), day 7 (I), and day 14 (J) in irradiated females after irradiation. Yellow arrows indicate PH3-positive cells. K Quantification of the average PH3-positive K. L–U Representative confocal microscopy images of ovarioles with Vasa (Green), cleaved-Caspase3 (Red), and DNA (Grey) staining at day 0 (L), day 1 (M), day 3 (N), day 7 (O), and day 14 (P) in controls, and at day 0 (Q), day 1 (R), day 3 (S), day 7 (T), and day 14 (U) in irradiated females after irradiation. Arrows point to somatic cells. The arrowhead points to the germ cell. The scale bar in A (for all image panels) is 50 µm. V Quantification of the cleave-Caspase3-positive somatic cells per ovariole. Error bars in K and V represent mean ± SEM. Comparisons of control and irradiated samples are performed by t-test; *** (P < 0.001), ** (P < 0.01), * (P < 0.05), ns (not significant). The number of samples in each group is shown above the X-axis