Fig. 5
From: Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti
X-ray irradiation does not interrupt the development of testis. A–J Representative confocal microscopy images of testes with Vasa (green), PH3 (red), and DNA (grey) staining at day 0 (A), day 1 (B), day 3 (C), day 7 (D), and day 14 (E) in controls, and at day 0 (F), day 1 (G), day 3 (H), day 7 (I), and day 14 (J) in irradiated males after irradiation. White arrows in C and D indicate mature sperm. Red arrows in H and J indicate Vasa-positive germ cells located at ectopic position (compared to G and I). The scale bar in A (for all image panels) is 50 µm. K Percentage of PH3-positive testes. Comparisons of control and irradiated samples are performed by Fisher’s exact test; **** (P < 0.0001), ns (not significant). The number of samples in each group is shown above each column