Fig. 6
From: Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti
X-ray irradiation affects sperm viability. A Quantification of sperm number in testes and seminal vesicle of wild-type and 30 Gy irradiated males. B Quantification of sperm number in spermathecae of wild-type females mated with wild-type or 30 Gy irradiated males. C Representative microscopy images of live sperm (Green) or dead sperm (Red) of wild-type and 30 Gy irradiated males. D Percentage of live sperm of wild-type or 30 Gy irradiated males. E-F Anti-H2Av staining only labels meiotic spermatocytes (red arrow) in wild-type testis. H2Av signals are also detected in GSCs, spermatogonia (white arrow), and differentiating spermatids (yellow arrow) in irradiated males (F). G–H TUNEL staining does not detect consistent signals in wild-type testes (G), but is elevated (decorating spermatid DNA, white arrow) in irradiated males (H). G’ and H’ are close-up views for the regions indicated in G and H. Comparisons of controls and irradiated samples are performed by paired t-test; *** (P < 0.001), ns (not significant). Error bars represent mean ± SEM. The number of samples in each group is shown above the X-axis