Fig. 3

Changes of senescence-related indicators of iMSCs in doxorubicin-induced acute senescence. A Representative morphology of iMSCs in doxorubicin incubation. Scale bar: 500 μm. B Mean cell area and length increased with doxorubicin duration. C Proliferation of iMSCs with doxorubicin incubation. D Scratch assay to detect iMSC migration in doxorubicin incubation. Scale bar: 1000 μm, the arrow indicated the width of the scratch. The mRNA levels of cell cycle genes (E), inflammatory cytokines (F), and stemness genes (G) of iMSCs with doxorubicin incubation. H The mitochondrial density of iMSCs with doxorubicin incubation. Scale bar: 200 μm. I The MMP of iMSCs with doxorubicin incubation. Scale bar: 200 μm. Data were representative of three independent experiments. n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by Student’s t test