Fig. 6

Cascade R-CNN system performance at senolytic-treated iMSC senescence. A The mitochondrial density of early and late senescent iMSCs with senolytic incubation. ABT-263, 2.5 μM; NMN, 200 μM; metformin, 100 μM. Scale bar: 200 μm. B The MMP of early and late senescent iMSCs with senolytic incubation. ABT-263, 2.5 μM; NMN, 200 μM; metformin, 100 μM. Scale bar: 200 μm. C Protocol for the Cascade R-CNN for detecting senolytic-treated late senescent iMSC data. D Output of the Cascade R-CNN to detect senolytic data. The Pearson correlation coefficient was calculated between the senescence proportional output of Cascade R-CNN and senescence-related indicators in the ABT-263 (E)/NMN (F)/metformin (G) incubation group. Data were representative of three independent experiments, n = 3