Fig. 2

Co-expression of Opn5B and Gq-rhodopsin in eye photoreceptors. a A schema explaining the cutting planes of the superior tentacle viewed from the right side. b An electron microscope image of the section of the retina that was cut at a similar position to the broken lines in (a), showing cross-sectioned rhabdomeres of the Type-I photoreceptors. Lower left panels (c, g) are immunofluorescence of Opn5B or Gq-rhodopsin. Next right panels (d, h) are fluorescence of streptavidin, which stains the apical protrusions of the Type-I eye photoreceptor. Some streptavidin signals are circumscribed with white circles to show similar distribution patterns between (d) and (h). (e) and (i) are the superimposed images of the left two panels on DAPI nuclear images. (f) and (j) are the bright-field images of the left panels. (c-f) and (g-j) are the pair of 6 μm-thick serial sections. Scale bar: (b) 10 μm, (j) 100 μm. A, anterior; P, posterior; D, dorsal; V, ventral; R, right; L, left. AR, accessory retina; AP, apical protrusion; MV, microvilli; PL, pigment layer; SA, streptavidin; BF, bright-field image