Fig. 3
Expression of G protein alpha subunits in the retina. a Immunostaining of limGαq. White arrows indicate the signals in the rhabdomeric region of the eye. b Fluorescence in situ hybridization with the antisense probe for limGαo mRNA. Arrow heads indicate the positive signals in the cell body layer. c Hybridization with the sense probe did not exhibit signals. d Immunostaining of limGαo showing the region near the eye. White arrows indicate the signals in the rhabdomeric region of the eye. e Pre-adsorption with MBP-limGαo protein diminished the immunoreactive signals. f A magnified image of (d) focusing on the rhabdomeric region (white arrows). Scale bars: 100 μm. TN, tentacular nerve; ON, optic nerve; BF, bright-field image. A, anterior; P, posterior; D, dorsal; V, ventral