Fig. 2

Localization of GPRC5C in the OE during regeneration of the OE. A Confocal images of OE cryosections of regenerating OE (3dpi, 14dpi and 28dpi), immunolabelled for GPRC5C (green) and GAP43 (red, left panel) and OMP (red, right panel). At 3dpi, no neurons were present and GPRC5C staining in the OE was not apparent. Dendritic knobs with GPRC5C expression can be seen at 14dpi and as regeneration of the neurons progresses, more knobs with GPRC5C staining are observed at 28dpi. B Quantification of GPRC5C positive knobs at 3-, 14- and 28-days post MMZ injection, counted in projections of confocal stacks of 16-μm thickness (n = 3 animals per group, individual data values Additional file 2, Student’s t test, error bars represent SEM, ***p < 0.001). C Confocal images of OE at 14dpi and 28dpi immunolabelled for GPRC5C (green) and γ-tubulin (red, left panel) and acetylated-tubulin (red, right panel). At 14dpi, GPRC5C + dendritic knobs have few newly formed acetylated-tubulin stained cilia, and at 28dpi, the amount of cilia increased. D Quantification of GPRC5C and γ-tubulin co-localization. Ca. 50% of the γ-tubulin + knobs express GPRC5C at 14dpi, at 28dpi ca. 80% of the dendritic knobs co-express GPRC5C and gamma-tubulin. Knobs were counted in projections of confocal stacks of 16-μm thickness, percentage co-expression was analysed using Student’s t test (n = 3 animals per group, individual data values Additional file 2, error bars represent SEM, **p < 0.01). E Z-stack projections of confocal images of WT and Gprc5c^−/− adult mouse OE cryosections showing comparable ARL13B staining. F Quantifications of staining intensity of ARL13B. Student’s t test showed no significant difference (n = 3, individual data values Additional file 2). Scale bars 10 µm