Fig. 5

Localization of ciliary markers in WT and Gprc5c^−/− OE. Z-stack projections of confocal images of WT and Gprc5c^−/− adult mouse OE cryosections immunolabelled for A ADCY3, B CNGA2, C PACS1 and D GNAL. Scale bar 10 µm. While GNAL (Gαolf) staining is comparable between WT and Gprc5c^{− −}ADCY3, CNGA2 and PACS1 ≥ is increased in Gprc5c^−/− cilia. E Quantifications of staining intensity of ciliary markers. Student’s t test showed significant increase in staining intensity for ADCY3, CNGA2, CNGA4, PACS1 and GNAL (n = 3–4, individual data values Additional file 2, error bars represent SEM, *p < 0.05, **p < 0.05). F Western blot analysis of ADCY3 expression in whole OE preparations, band slightly above 180 kDa, equal loading was controlled by actin. G Confocal images of en face preparation of septal OE of adult WT and Gprc5c^−/− mice immune-labelled for Or5d18, depicting the anterior region. Scale bar: 10 μm. H In the posterior region, cilia are in general shorter compared to the anterior region. I Violin plots of length of Or5d18 cilia in WT and Gprc5c^−/− mice. K Quantification of the length of Or5d18 + cilia per OSN. Student’s t test showed significant increase in Gprc5c^−/− OE (n ≥ 50 knobs, error bars represent SEM, ***p < 0.001). L Quantification of staining intensity of posterior mOR-EG neurons in WT and Gprc5c^−/− OE. Student’s t test showed significant increase in Gprc5c^−/− OE (n = 3, individual data values Additional file 2, error bars represent SEM, * p < 0.05). M Increased localization of RAB11-positive structures in the dendritic knobs of Gprc5c^−/− mice (n = 4 animals per group, individual data values Additional file 2, Student’s t test, error bars represent SEM, *p < 0.05). N Z-stack projections of confocal images of WT and Gprc5c^−/− OE cryosections immunolabelled for RAB11. Scale bar 10 µm