Fig. 6

Redistribution of ciliary phospholipids in Gprc5c^−/− OE. Confocal images of WT and Gprc5c^−/− adult mouse OE cryosections immunolabelled for A PtdIns(4,5)P2 and B PtdIns4P. PtdIns(4,5)P2 staining is restricted to the dendritic knobs in WT OE whereas, Gprc5c^−/− OE shows several prominent puncta in the ciliary layer. PtdIns4P on the other hand showed a notable reduction in the Gprc5c^−/− cilia. C Quantification of PtdIns(4,5)P2 staining intensity. Student’s t test showed significant increase in staining intensity (n = 3, individual data values Additional file 2, error bars represent SEM, **p < 0.01). D Average expression and percent of respective OE cell types expressing different (putative) 5-phosphatases, OSNs are highlighted, data taken from [55]. HBC-horizontal basal cell, GBC-globose basal cell, INP-immediate neuronal progenitor cell, iOSN-immature olfactory neuron, OSN-mature olfactory neuron, MV-microvillar cell, SUS-sustentacular cell. Scale bars: A, B 10 µm