Fig. 7

Effects of GPRC5C knockout on neuronal activity and behaviour. A Quantification for buried food test. Gprc5c^−/− mice exhibit a significantly reduced latency (WT 395.4 ± 63.66 s, Gprc5c^−/− 226.5 ± 36.49 s) to find buried food compared to WT mice, indicating an altered behavioural response (n = 18 animals per group, Student’s t test, error bars represent SEM, * p < 0.05). B Tile scans of the septum of the OE stained for Na/K + ATPase, both images were taken with the same microscope settings. C Z-stack projections of confocal images of WT and Gprc5c^−/− adult mouse OE cryosections immunolabelled for PS6. D Quantification of staining intensity of PS6 neurons in WT and Gprc5c^−/− OE Student’s t test showed significant increase in Gprc5c^−/− OE (n = 3, individual data values Additional file 2, error bars represent SEM, *p < 0.05). E Quantification of number of PS6 neurons. Student’s t test showed significant increase in Gprc5c^{− −}E (n = 3, individual data values Additional file 2, error bars represent SEM, ***p < 0.001). F Quantification of the number of mitochondria per dendritic knob (n = 155 WT, n = 162 Gprc5c^−/−). Mann–Whitney test shows no significant difference. G TEM of ultrathin sections of WT and Gprc5c^−/− OE depicting altered mitochondria in dendritic knobs of Gprc5c^−/− OE. Scale bar 400 nm. H Quantification of MT-CO1 staining as mitochondrial marker. Student’s t test shows significant reduction in staining intensity of MT-CO1 in dendritic knob layer of Gprc5c^−/− OE (n = 3, individual data values Additional file 2, error bars represent SEM, **p < 0.01). I Z-stack projections of confocal images of WT and Gprc5c^−/− adult mouse OE cryosections immunolabelled for MTCO1 (red) showing reduced staining in dendritic knobs of Gprc5c^−/− OE. Scale bar 10 μm