Fig. 1

DNAJB6 knockdown increases tau aggregation. A The filter trap assay detected tau aggregation. The cellular lysate of SH-SY5Y neuroblastoma cells transfected with the empty vector (pEGFP-C1), wild-type tau, or mutated tau P301L for 48 h was filtered through cellulose acetate membranes. The dot blot assay was then performed with a tau antibody to detect trapped tau. B Quantification of tau aggregates. The values were given as mean ± standard deviation (S.D.) (n = 3, *P < 0.05, unpaired two-tailed Welch’s t-test). C tau expression level of cells in A was confirmed by immunoblotting. β-actin was used as a loading control for the normalization in B. D shLuc or shDNAJB6 SH-SY5Y cells were transfected with an empty vector or tau P301L for 48 h. The cellular lysate was filtered through cellulose acetate membranes and retained proteins were stained with a tau antibody. E Quantification of tau aggregates. The values were given as mean ± S.D. (n = 3, *P < 0.05, **P < 0.01, unpaired two-tailed Student’s t-test). F Expression of tau and knockdown of DNAJB6 in cells shown in D were determined by immunoblotting using tau and DNAJB6 antibodies, respectively. β-actin was used as a loading control for the normalization in E. The individual data values of the replicates in B and E are listed in Additional file 2