Fig. 3

Overexpression of DNAJB6 isoform b reduces tau aggregation. A A diagram of various domains of DNAJB6 protein. The alternatively spliced C-terminal parts of isoforms a and b are indicated. DNAJB6a and DNAJB6b share the N-terminal 231 amino acids but differ in their C-terminal region and their cellular localization. DNAJB6a, the longer isoform, contains an NLS and predominantly localizes in the nucleus, whereas the shorter DNAJB6b primarily localizes in the cytoplasm. B The cellular lysate of SH-SY5Y cells which co-transfected an empty vector or mutated tau P301L together with an empty vector (pcDNA/FRT/TO-V5), V5-DNAJB6a, or V5-DNAJB6b for 48 h was filtered through cellulose acetate membranes. Retained proteins were stained with a tau antibody. C Quantification of aggregation tau. The values were given as mean ± S.D. (n = 3, *P < 0.05, N.S. non-significant, unpaired two-tailed Student’s t-test). The individual data values of the replicates in C are listed in Additional file 2. D The expression levels of tau and DNAJB6 were determined by immunoblotting using tau and DNAJB6 antibodies, respectively. β- actin was used as a loading control for the normalization in C