Fig. 4
From: The HSP40 family chaperone isoform DNAJB6b prevents neuronal cells from tau aggregation
Expression of DNAJB6b decreases visualized tau aggregation in neuroblastoma cells. A Representative fluorescent images of BiFC and DNAJB6 in SH-SY5Y cells that co-expressed the BiFC-tau P301L constructs and an empty vector, V5-DNAJB6a, or V5-DNAJB6b. The fluorescence emitted by Venus complementation was imaged 48 h after transfection. Cells were immunostained with DNAJB6 and counterstained with DAPI. Enlarged Venus images of the representative cells corresponding to the selected regions are shown. Scale bar: 20 μm. B The expression levels of BiFC-tau P301L and DNAJB6 were indicated by immunoblotting with tau and DNAJB6 antibodies in SH-SY5Y cells, respectively. C Quantification of BiFC-fluorescence of A. The percentage of BiFC-positive SH-SY5Y cells to the total number of cells was quantified. The values were given as mean ± S.D. (n = 3, **P < 0.01, unpaired two-tailed Student’s t-test). The individual data values of the replicates in C are listed in Additional file 2