Fig. 4

CG5846 is sequestered into HDAC4 aggregates but its expression does not alter the number of aggregates nor mushroom body phenotypes. A Immunohistochemistry on brains expressing OK107-GAL4 driven Myc-tagged HDAC4^WT or HDAC4^ΔANK (green) in the presence and absence of HA-tagged CG5846 (magenta) in the mushroom body. Flies were raised at 23 °C. Representative maximum projections of 0.5-μm sections through the Kenyon cell bodies are shown. Scale bar = 50 μm. B Quantification of nuclear aggregates. Aggregates were counted through the maximum projection across the entire Kenyon cell layer, n = 4 brains/genotype. The number of aggregates was significantly increased in the Kenyon cell nuclei of HDAC4^ΔANK and HDAC4^ΔANK;CG5846 brains in comparison to HDAC4^WT;CG5846 (ANOVA, F_(3,12) = 9.03, p = 0.0021, Tukey’s post hoc test, **p < 0.01), but there was no significant difference between HDAC4^WT and HDAC4^WT;CG5846, nor HDAC4^ΔANK and HDAC4^ΔANK;CG5846. C Nuclear aggregates were quantified as in B. There was no significant change in aggregate number when CG5846 was knocked down (ANOVA, F_(2,9) = 0.98, p = 0.412). D Quantification of mushroom body phenotypes. The percentage of brains displaying each phenotype is shown. There was no significant difference in the proportion of brains displaying defects between those expressing HDAC4^WT, HDAC4^WT;CG5846, HDAC4^ΔANK and HDAC4^ΔANK;CG5846 with the elav-GAL4 driver