Fig. 6

Expression of nuclear and cytoplasmic-restricted HDAC4 exacerbates disruption to eye development, which is reduced in the Y1142H mutant. A Stereomicrographs of Drosophila eyes. Genotypes were generated by crossing GMR-GAL4/CyO;UAS-HDAC4 females to males carrying each UAS-HDAC4 transgene and to the w(CS10) control and collecting non Cy progeny. Representative images of n = 5 males and n = 5 females per genotype are shown. × 110 magnification. B Quantification of the severity of eye phenotypes. In comparison to HDAC4^WT, the severity of HDAC4^3SA, HDAC4^ΔANK, and HDAC4^ΔNLS phenotypes was significantly increased. In contrast, the HDAC4^ΔMEF2 and HDAC4^Y1142H phenotypes were both significantly reduced in males and females (females: ANOVA, F(8,36) = 218.5, p = 1.11 × 10⁻¹⁶. Tukey’s post hoc test, **p < 0.01; males: ANOVA, F(8,36) = 157.58, p = 1.11 × 10⁻¹⁶. Tukey’s post hoc test, **p < 0.01). Error bars = mean ± SEM. C Anti-Myc immunohistochemistry on eye imaginal discs from wandering third instar larvae expressing HDAC4^WT and mutants with GMR-GAL4. Representative images from n = 15 discs per genotype are shown. Scale bar = 10 μm